Long Noncoding RNA as Modular Scaffold of Histone Modification Complex
Long intergenic non protein coding rna is a class of long non coding rna in plants molecule with a length of more than 200nt, which is a by-product of RNA polymerase II transcription. It can regulate gene expression at various levels (epigenetic regulation, transcriptional regulation and post-transcriptional regulation, etc.). It is a research hotspot of genetics.
Research Scheme of Lncrna Scaffold System Based on ceRNA Regulatory Mechanism of Hanbio
To verify the expression level and intracellular sublocalization of lncRNA;
Predict the miRNAs that lncRNAs may bind to through network database;
Informatic screening of lncRNA-bound miRNAs and further screening and validation by luciferase assay;
miRNA target gene prediction and functional enrichment analysis;
Screening, verification and related functional studies of miRNA target genes;
Verification of lncRNA-miRNA-mRNA regulatory network.
Han bio Validation Services Advantages on lncRNA and Target Gene Competitive Binding microRNA
Professional software was used to predict the binding sites of miRNA, lncRNA and target genes;
psiCHECK plasmid unique to Promega Company was adopted;
The Luciferase dual fluorescence reporting system can obtain a wide linear quantitative range and extremely high sensitivity;
The instantaneous transfection technique is simple, rapid and short period, and is suitable for most cell types.
