HIV Gene Therapy — Self Experimental Protocol (N6 v2)

Summary: In late 2017, I tested out a gene therapy that targets HIV. The describes the protocol used for testing out a similar yet different gene vector, as well as an update following the first week.

As described in earlier articles, this 2nd version is designed to deliver the gene for N6, an antibody that binds to HIV’s GP120 protein. This 2nd version (N6 v2) has the bacterial elements removed from the plasmid, creating a ‘mini circle’ that may last for months if not years in one’s body.

We expect to have data from this experiment in May.

Safety: One of my regrets from the first test was that I did not wait longer in between the ‘test’ dose, and the ‘real’ dose — only about an hour between injections. This time, I waited a number of days, giving me greater confidence that my body was not going to flip its shit when it received the full injection.

Test dose: March 10th, 2019. 700 nanograms of plasmid (with PEI and saline). A small red bump materialized that was similar to the 2017 injection. No other symptoms seemed to occur.

Full dose: March 16th, 2019. 10 micrograms + 40 micrograms PEI +11 microliters saline.

Dependent Variable: Binding to GP120 in blood serum. In other words: is my blood blocking HIV more after the injection? I will be gathering samples at least once a week, and for those of you who haven’t drawn their own blood, this is not a particularly fun or easy process.

After two months of data collection, we will send the samples to a third party to quantify the results. Success would mean that the expression of the antibody is sustained after the initial plateau is reached.

The rest of this article has been temporarily removed until we’ve isolated the variable that caused the negative reaction. Sorry. ><