Welcome to the 47th part of the multi-part series on applications of CRISPR in gene editing and beyond. The ability to precisely edit genomic DNA using the CRISPR-Cas system has revolutionized the field of genome engineering. Like prime editing, base editing is a CRISPR-based precise genome editing technology that can…

Welcome to the 46th part of the multi-part series on applications of CRISPR in gene editing and beyond. Undoubtedly CRISPR-Cas technology has changed the way scientists approach gene editing, offering various advantages that were never seen before. Scientists are able to edit human, animal, and plant genomes with this technique…

Welcome to the 45th part of the multi-part series on applications of CRISPR in gene editing and beyond. What are gene drives? Gene drives are self-propagating genetic elements that rapidly promote the inheritance and spread of the desired gene variant throughout a population. In the traditional Mendelian inheritance — ­each allele has a 50%…

Welcome to the 44th part of the multi-part series on applications of CRISPR in gene editing and beyond. CRISPR-Cas9 tool can also act as antiviral therapy in treating viral infections like: Human Immunodeficiency Virus or HIV HIV belongs to the retrovirus family of viruses and stores its genetic information using single-stranded RNA. The outer shell…

Welcome to the 43rd part of the multi-part series on applications of CRISPR in gene editing and beyond. Adoptive cell therapy, abbreviated as ACT, is a type of immunotherapy which seeks to harness immune cells, particularly T cells, for fighting cancer. Various adoptive cell therapies are: (i) Tumor-infiltrating lymphocyte (TIL)…

Welcome to the 42nd part of the multi-part series on applications of CRISPR in gene editing and beyond. Cationic lipids/lipoplexes Cationic lipids are very important non-viral lipid vectors used in gene editing and gene therapy purposes because they can be easily synthesized and modified. The cationic lipids contain positively charged hydrophilic heads…

Welcome to the 41st part of the multi-part series on applications of CRISPR in gene editing and beyond. Until now, we have understood that the viral vectors (Parts 34, 35, 36, 37, 38, 39, and 40) are crucial for transferring the CRISPR system components gRNA and Cas9 into the target…

Welcome to the 40th part of the multi-part series on applications of CRISPR in gene editing and beyond. The third type of viral vectors used for CRISPR-mediated gene editing purposes are lentiviral vectors, derived from human immunodeficiency type-1 (HIV-1) lentivirus. Adenoviral Vectors for delivery of CRISPR components (Part 35- CRISPR in gene editing and beyond) Welcome to the 35th part of the multi-part series on applications of CRISPR in gene editing and beyond.medium.com

Welcome to the 39th part of the multi-part series on applications of CRISPR in gene editing and beyond. The second type of viral vectors used for CRISPR-mediated gene editing are adeno-associated viral vectors, abbreviated as AAV vectors. The adeno-associated virus was initially discovered as a contaminant of adenovirus preparations; hence…