The regulation mechanism of LSD1 in somatic cell reprogramming process

Recently, the journal Scientific Reports published the latest research results of Zheng Hui study group from Guangzhou Institute of Biomedicine and Health and Chinese Academy of Sciences online, and the paper is titled “Lysine-specific histone demethylase 1 inhibition promotes reprogramming by facilitating the expression of exogenous transcriptional factors and metabolic switch”. The research, using various recombinant proteins including recombinant dog proteins, reveals the regulation mechanism of histone lysine residues methylation enzyme LSD1 on cell metabolism and the transcription factor expression in somatic cell reprogramming process.

Epigenetic refers to heritable changing process of gene expression the process of cell division without altering DNA sequence. While in the process of cell reprogramming, epigenetic information within cells has changed, and especially histone methyl glycosylation state has undergone tremendous changes. As a member of epigenetic modification enzymes, LSD1 can specifically modify histone lysine residues H3K4 and H3K9 methylation status, but the study reports of its mechanism in the reprogramming process are relatively less.

Zheng Hui study group used MEFs and pre-iPSCs cells as model system to systematically describe the gene expression of LSD1 to exogenous transcription factors in reprogramming process and its impact on the transformation of metabolic way in reprogramming process. The researchers found that in the reprogramming process of somatic cells, inhibiting the activity of LSD1 can improve the efficiency of cell reprogramming. Further study found that inhibiting the activity of LSD1 can prevent the H3K4 demethylation of upstream area of exogenous transcription factor gene, thereby improving the expression of endogenous transcription factor, but also speed up the transformation of metabolic way in reprogramming process by affecting the expression of Hif1α gene and its downstream genes, thus improving the efficiency of reprogramming process. Finally, in the process of pre-iPS cells transforming into iPS cells, inhibiting the activity of LSD1 also can significantly improve the conversion efficiency of the pre-iPS cells by changing their metabolism way.

The research was mainly conducted by Zheng Hui study group and Guo Yunqian from Beijing Forestry University. Related achievements were funded by the National Natural Science Foundation of China, Chinese Academy of Sciences, Municipal and Technology project of Guangzhou. Flarebio provides you with good-quality recombinant proteins such as recombinant Cdh3 at competitive prices.