How to Make Your Own Chemically-Competent Cells

I once had the terrible experience of not being able to run an assay because I ran out of commercial stock of transformation-competent Escherichia coli (E.coli). From that day, I learned to make my own chemically-competent cells in the lab. I recommend that everyone makes their own stash of transformation-competent E.coli stocks — among other suggested laboratory […]

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