Igor Dzhura — A Medical Professional with Exceptional Research Skills
Igor Dzhura is a successful medical professional with extensive experience in supporting research in biochemistry, cancer research, microbiology, and drug recovery. He has a slew of research skills, which helped him build a successful career in the medical field. He has comprehensive knowledge of basic molecular biology methods — PCR, qPCR and RT-PCR; cloning: digestion, ligation, transformation; application of adenovirus, lentivirus and plasmid expression vectors; reparation and characterization of cellular proteins (whole cell and subcellular lysate preparation, cell fractionation, SDS-PAGE, immunoblotting and immunoprecipitation) DNA/RNA/cDNA isolation (blood, FFPE, fresh tail, maxi-mini preps); electrophoresis etc.
In addition, Igor Dzhura is also experienced in:
- Glucose stimulated insulin secretion assay, ELISA assay.
- Primary cell culture: Isolation of cardiomyocytes, isolation of islets of Langerhans from mice/rat pancreas: isolation of single β-cells, iPSC (stem cells).
- Bacterial and mammals cell culture: COS-7, HEK293, MIN-6, tSA-201,HB101, AGS, INS-1, MKN (feeding, splitting, and cryopreservation).
- Flowcytometry: BD FACSCanto II, BDFACSCanto
- Automated electrophysiology : Qpatch, Quattro, Barracuda
- Conventional electrophysiology: whole-cell and single channel patch clamp (in different configurations), Dynaflow system
- Langendorff Heart System
- Bilayer recording
- Calcium imaging, FLEX, FRET.
- FLIPR, Hamamatsu7000, TECAN/Hamilton
- Biostatistics: work with database (ANOVA, Fisher test, t-test)
- Working experience under GLP, CLIA, CAP
Igor Dzhura is a well-educated professional with a Master of Science and a Bachelor degree in Biology/Chemistry from National Kiev University. He is exceptional troubleshooting, problem solving, and team handling capabilities. He is a dedicated, diligent professional who has successfully accomplished a variety of projects on medical research.
Igor Dzhura’s very first job was at the Bogomoletz Institute of Physiology in Kyiv where he worked from 1998 to 2000. He studied T-type (Cav3.2) channels: regulation, properties, pharmacology (electrophysiology, RNA/DNA isolation, cell culture, etc.). Then, he worked for the SUNY Department of Medicine and Joslin Diabetes Center in Syracuse, NY for two years. Here at this center, he served as a Senior Research Associate, and performed various duties:
- Modulators of β-cell glucose signaling in Diabetes using following methods: e-phys, calcium imaging, isolation of islets of Langerhans and β-cells from mice/rat pancreas, ELISA assay, Glucose stimulated insulin secretion assay, SDS-PAGE, Western Blot.
- Study of calcium signaling in pancreatic β-cells. Investigate the localization and characterization of specific proteins. In this research using fluorescent and confocal microscopy, siRNA/shRNA lentiviral transduction, creating genetically encoding biosensors.