Igor Dzhura has a Great Deal of Experience as a Medical Researcher

Igor Dzhura is a medical specialist who has a Masters degree in Science, which he completed in 1998 from National Kiev University, Kyiv, Ukraine. Prior to this he did a bachelor degree in Biology/Chemistry from the same university in 1995. He has a wealth of knowledge and experience in different medical research areas. These include:

Basic molecular biology methods: PCR, qPCR and RT-PCR; cloning: digestion, ligation, transformation; application of adenovirus, lentivirus and plasmid expression vectors; reparation and characterization of cellular proteins (whole cell and subcellular lysate preparation, cell fractionation, SDS-PAGE, immunoblotting and immunoprecipitation) DNA/RNA/cDNA isolation (blood, FFPE, fresh tail, maxi-mini preps); electrophoresis etc.

Glucose stimulated insulin secretion assay, ELISA assay.

Primary cell culture: isolation of cardiomyocytes, isolation of islets of Langerhans from mice/rat pancreas: isolation of single β-cells, iPSC (stem cells)

Bacterial and mammals cell culture: COS-7, HEK293, MIN-6, tSA-201,HB101, AGS, INS-1, MKN (feeding, splitting, and cryopreservation).

Flowcytometry: BD FACSCanto II, BDFACSCanto

Electrophysiology:

o Automated electrophysiology : Qpatch, Quattro, Barracuda

o Conventional electrophysiology: whole-cell and single channel patch clamp (in different configurations), Dynaflow system

o Langendorff Heart System

o Bilayer recording

o Calcium imaging, FLEX, FRET.

o FLIPR, Hamamatsu7000, TECAN/Hamilton

Biostatistics: work with database (ANOVA, Fisher test, t-test)

Microbiology, Biochemistry, Cancer Research, Ion Channels Regulation and Drug Discovery are the areas in which Igor Dzhura has expertise. He is a dedicated, driven professional who has excellent problem solving and medical research and troubleshooting skills. Since the year he started his career, he has handled a number of research projects so far. He worked for Bogomoletz Institute of Physiology for two years, followed by Research Associate at Vanderbilt University Medical Center in Nashville, TN.

From 2008 to 2010, Igor Dzhura was employed at SUNY Department of Medicine & Joslin Diabetes Center, Syracuse NY as a Senior Research Associate. It is all due to his dedication and hard work that he became able to earn promotion to this position. His responsibilities were revised to:

Modulators of β-cell glucose signaling in Diabetes using following methods: e-phys, calcium imaging, isolation of islets of Langerhans and β-cells from mice/rat pancreas, ELISA assay, Glucose stimulated insulin secretion assay, SDS-PAGE, Western Blot.

Study of calcium signaling in pancreatic β-cells. Investigate the localization and characterization of specific proteins. In this research using fluorescent and confocal microscopy, siRNA/shRNA lentiviral transduction, creating genetically encoding biosensors.

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