Cell free DNA extraction from blood
Cell free DNA can found in the bloodstream. This cell free DNA can be captured as a biological sample for disease analysis such as serum or blood. Analyzing cell free DNA is useful for a wide range of research applications such as next generation sequencing, digital PCR, and real-time PCR. An analysis kit is designed for enrichment of small circulating DNA that is present in the biological samples. Such a kit can use magnetic bead technology in order to ensure high-quality DNA’s reproducible recovery.
Cell free DNA from blood can be used for analyzing various diseases, including cancer. Two major issues could be encountered by researchers who need to analyze circulating tumor DNA: the relatively low amount of DNA in circulation and the white blood cells’ gDNA contamination. For these reasons, it is important to use proper protocols for circulation DNA extraction and blood collection. Several kits are available on the market for this purpose, providing different performances and various levels of impact of blood processing and collection protocols.
Such an analysis kit features a magnetic bead–based purification format that allows scientists and lab technicians to process easily a various sample input volumes. When used with the a Flex Magnetic Particle Processor, can be isolated and processed up to 24 plasma/serum 2 mL samples of input volume. With the use of a magnetic stand, these samples can be also processed manually.
The Isolation Kit offers you the following features:
• Fast procedure allowing processing of a range of 6 to 24 samples in just maximum 40 minutes time
• Phenol free and automation ready extraction
Performing manual and automated purification from plasma samples is an easy task. With the enrichment of cell free DNA, scientists can unlock various useful information. Isolating and analyzing such a sample from urine, serum or blood has the potential to provide a non-invasive approach to monitoring and detection of targets associated with cancer and other disease states.
Efficient nucleic acid isolation is the first step in attaining this valuable information. This leaves the larger DNA molecules behind while it recovers the circulating cfDNA. This enrichment aspect for the cfDNA portion of the nucleic acid makes sure that the fragmented and shorter DNA is ready for downstream analysis using next generation sequencing, digital PCR and real-time PCR.
The cell free DNA extraction kit is designed for both automatic and manual isolation and extraction of cfDNA from a various samples in the volume input range from 0.5 mL to 10 mL. The magnetic bead format is phenol-free and makes it fully possible that the kit works with automated isolation.
Due to the scalable format suitable for different sample inputs, the number of processed samples can vary with a single kit. For instance, each kit provides sufficient reagents to process 50 samples of 2 mL serum or plasma each or 25 samples of 4 mL serum or plasma each. If necessary, the input volumes can be scaled down or scaled up in order to change the total number of processed samples. Thanks to this feature, scientists can tailor the procedure to various requirements and needs.