Bioremediation of crude oil impacted soil utilizing surfactant, nutrient and enzyme amendments — JBES

Samson Eruke Okoro Akuro Adoki

Department of Biochemistry, University of Port Harcourt, P.M.B 5323, Port Harcourt, Nigeria

Oil Spill Response & Remediation Department, Shell Petroleum Development Corporation, P.O. Box 263, Port Harcourt, Nigeria

Key words: Bioremediation, biostimulation, surfactant, bioremediation agents.


The present study was designed to investigate possible methods to enhance the rate of biodegradation of crude oil highly impacted soil excavated from a site at K-Dere in Gokana Local Government Area of Rivers State, Nigeria. The experiment consisted of fourteen treatment reactor vessels subdivided into Groups EA, UA, SD, EUS, SLG-GS and SLG and set up in triplicate concentrations. Individual reactor vessels contained 20kg of crude oil impacted soil and 7kg of agricultural soil. Groups EA, UA, SD and EUS were treated with an anionic surfactant sodium dodecyl sulfate (SDS), enzyme additive, oleophilic nutrient (uric acid) and a combination of SDS, enzyme additive, uric acid respectively. Control group SLG did not receive any treatment while Control group SLG-GS received agricultural soil only. TPH reduction in the impacted soil varied between 4.0 and 45.0% within five weeks as well as between 11.0 and 64.0% after nine weeks of applying the treatments respectively. The group that received a combination of SDS, enzyme additive and uric acid showed the highest reduction in TPH by the end of the ninth week. Total Organic Carbon (TOC) reduction ranged from 4.0 and 30.0% between day 0 and week 5 as well as from 0.0 and 50.0% between weeks 5 and 9 respectively in the test groups. The pH of the degrading impacted soil fluctuated between 6.12 and 9.49 during the same period. When compared with the 0.0% TPH reduction in the Control group SLG, the added treatments evidently increased he rate of bioremediation of the impacted soil.

Get the original articles in Source: Volume 4, Number 4, April 2014 — JBES

Journal Name: Journal of Biodiversity and Environmental Sciences (JBES)

Published By: International Network for Natural Sciences

Related Post: Genetic diversity and phylogeography of hawksbill turtle in the Persian Gulf — JBES