Glyphosate resistance trait into soybean Cuban varieties: agronomical assessment of transgenic lines until F6 generation — IJAAR
Celia Delgado, Gil A. Enríquez, Rodobaldo Ortiz, Odett Céspedes, Natacha Soto, Yuniet Hernández, Merardo Pujol
Centre for Genetic Engineering and Biotechnology, P. O. Box 6162, Havana 10600, Cuba
National Institute of Agricultural Sciences, Apdo 10, San José De Las Lajas, Mayabeque 32700, Cuba
Key words: Soybean, Glyphosate resistance, GTS 40–3–2 event, Introgression, Transgenic lines.
Glyphosate-resistant (GR) soybean was one of the first major applications of genetic engineering in field crops and offered farmers a vital tool in fighting weeds. Weeds are a problem for soybean production in Cuba, so our work aim was the GTS 40–3–2 event introgression into Cuban varieties. Two local cultivars were crossed with transgenic genotypes that carry the event. From F1 to F3 generations, individual plants that produced more than 60 g of seeds per plant were chosen to obtain next generation. Individual lines were selected from F4 generation. F5 and F6 generations of five selected transgenic lines and their relatives were chosen to evaluate seven agronomic traits throughout the summers of 2012 and 2013.
A Random Block experimental design was done. First flowering (R1) and maturity (R8) stages of all genotypes were affected by planting date. Plant height of I1B2- 3, I1B4, I36B4 and RP5 lines ranged from 80 to 111 cm. I1B2–2 and I1B2–3 lines would be suitable for mechanized harvesting because they had the insertion of the first pod at 14.63 cm and 13.93 cm respectively. I36B4 line produced the greatest number of pods per plant (127). Transgenic lines produced more than 180 seeds per plant and 100-seed weight ranged from 13.75 g to 17.46 g. Seed yield per plant of transgenic lines and their parents IncaSoy36, CEB2 and CEB4 weren’t statistically different. These results could be a start point for other studies involving larger areas, different planting dates and localities.
Get the original articles in Source: Volume 7, Number 4, October 2015 — IJAAR
Published By: International Network for Natural Sciences