Jul 11, 2016

In vitro plant regeneration from male flowers of banana

Md Tipu Sultan, Mahboob Hossain Khan, Md Lokman Hakim, ANK Mamun, Md Alam Morshed, Md Rofiqul Islam, Md Rokibul Islam
Department of Biotechnology and Genetic Engineering, Faculty of Applied Science and Technology, Islamic University, Kushtia 7003, Bangladesh.
Institute of Food and Radiation Biology (IFRB), Atomic Energy Research Establishment (AERE), Savar, Dhaka, Bangladesh.
Department of Pharmacy, North South University, Bashundhara, Dhaka 1229, Bangladesh.

Key words: Musa sp. cv. Sabri, indirect organogenesis, callus, plyploids, somaclonal variation

Abstract
The present study was undertaken with a view to establish a protocol on in vitro regeneration of plants by using young male flowers of banana (Musa sp. cv. Sabri) as explant. Appropriate developmental stage of immature male flowers for inoculation, medium composition for induction of calli, regeneration of plants, rooting of in vitro regenerated shoots, acclimatization of in vitro regenerated plantlets and ex vitro establishments of plantlets were worked out. Young male flowers obtained by striping away the bracts in between 24 to 26 were found suitable as explants for induction of callus. The isolated male flowers were cultured on MS (Murashige and Skoog) medium supplemented with different concentrations and combinations of three auxins viz. 2, 4-D (2,4-Dichlorophenoxyacetic acid), NAA (Napthaleneacetic acid) and IAA (Indole-3-acetic acid) for induction of callus. Only two of the total twenty two medium composition yielded calli. The better response (20%) was recorded in MS medium containing 2.0 2, 4-D + 0.5 NAA + 0.5 IAA (Indole-3-acetic acid) (mg/l).

The calli were cultured on MS medium fortified with different concentrations of BA (Benzyladenine), NAA, IAA and Glutamine or Caesin hydrolysate (CH) to regenerate shoots. MS medium having the supplementation of 1.0 BA + 0.5 IAA + 500 CH (mg/l) was appeared best for regeneration of shoots. Single isolated regenerated shoots were implanted on MS medium supplemented with three different concentrations (0.5, 1.0 and 2.0 mg/l) of IBA or NAA to induce root. IBA at a concentration of 1.0 mg/l produced best rooting. The plantlets were gradually acclimatized and transferred to the soil. The survival percentage was about 90%.

Posted By: Volume 1, Number 1, February 2011- IJB

Publish Article: International Journal of Biosciences (IJB)