Electroporation Instruments Are Used As An Electric Pulse To Introduce Foreign Genes Into Cell Membranes In Gene Therapies
An electroporation instrument is a tool that allows scientists to introduce foreign genes into cells. Electroporation is a method of physical transfection that uses a low-level electric field to create a temporary hole in cell membranes. These tiny holes allow chemical or genetic materials to enter cells. This method is often used to introduce foreign genes into cells. Electroporation is also a good way to produce high-quality protein expression in cell culture. Electroporation instruments are used in various end-user applications such as biotechnology or pharmaceutical industries, hospital laboratories, and research institutes.
While Electroporation Instruments are technically possible at a high-throughput scale, it has been slow to catch on in the scientific community. Commercial electroporation instruments require expensive plates and buffers that require proprietary methods. As a result, they are unsuitable for large-scale screening. Moreover, these instruments are not designed for high-throughput applications. Therefore, it is important to consider these factors before making a purchase.
A typical high-throughput electroporation system allows researchers to perform transfection experiments with thousands of siRNAs. Its high-throughput capabilities can even make it possible to use miniaturized electroporation instruments to perform transfections. These instruments also enable scientists to perform cell culture experiments in a 96-well plate. And if they can’t optimize a cuvette-type electroporation device, it can be used to develop high-throughput transfection systems. For instance, in January 2022, a research team at the Max Planck Institute for Developmental Biology, Germany, conducted a study on mutations in damaged DNA to create new variations using electroporation instruments.
As the number of gene editing applications grows, researchers are increasingly integrating advanced gene editing procedures into their experiments. These techniques involve curating targeted sequence-specific alternations within DNA. Examples of these advanced technologies are CRISPR-associated proteins, clustered regularly-interspaced short palindromic repeats (CRISPR), and transcription activator-like effector nucleases. Given the growing demand for gene-editing tools, electroporation instruments are sure to become more popular in the coming years.