BHI Medium stands out as a valuable growth medium catering to a diverse array of microorganisms due to its exceptional nutritive composition. Moreover, it serves as a pivotal tool in preparing inocula for assessing antimicrobial susceptibility. Stemming from Rosenow’s original concept, BHI Broth is an evolved variant where dextrose broth was enriched with fragments of brain tissue. This adaptation not only retains the nutritive richness but also enhances its buffering capacity, thereby fostering the growth of a wide spectrum of organisms. Particularly well-suited for anaerobic bacteria, yeasts, and molds, BHI Broth becomes even more versatile with the incorporation of 10% defibrinated sheep blood. This addition facilitates the isolation and cultivation of Histoplasma capsulatum and other fungi. To selectively target fungi, the introduction of gentamicin and/or chloramphenicol is recommended as an enhancement to the medium’s effectiveness. Read More..
