Hi Zeynep,
Sun-Gou Ji
1

I see. Thanks a lot for the response, Sun-Gou! I learned that in the “normalized” ROS/MAP data on Synapse, the batches of plates 1–6 and plates 7–8 were batch corrected separately for individual plates (e.g. plates 1–6 and 7–8 respectively), but not across all plates. I think that’s why plates 7–8 can still be separated from plates 1–6 in the PC1-PC2 plot. I think I should correct one more time for all plates using the normalized data.

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