An epigenetic age reduction world record?
After eight months of Blueprint…
I reduced my epigenetic age by 5.1 yrs in 7 months.
That’s .73 years of age reversal, per month, using multi-epi-clock average.
The Rejuvenation Olympics, where you win by never crossing the finish line.
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It’s possible that, this month, the Blueprint team set a new world record in reversing an aging biomarker: my epigenetic age. Specifically, we recently saw a large reduction in my multi-epi-clock average, per unit time, from age 47 to 42.5 in seven months. That’s over six months of age reversal, per single month, without the use of growth hormone. One step forward, six steps back.
To our knowledge, according to published data, this is a world record.
These results were achieved via Blueprint’s measurement and intervention protocols, part of an endeavor that aims to measure all my 78 organs and then reverse the age of each. (Blueprint basics is a summary of my daily wellness protocol. The Blueprint website includes my progress reports on this journey.)
For comparison, the TRIIM trial, a multi-drug, anti-aging clinical trial, showed an average reduction in epigenetic age by 0.9 years at 9 months (or, a little over of month of reversal per month) across 4 epigenetic clocks (Horvath, Peno, Hannum, Grim); these are changes in DNA methylation patterns that can reliably predict chronological and biological age.
Another trial, in April 2021 tested an intervention against a single Horvath pan tissue clock, showing 1.96 years reduction in this single clock on average after 2 months, However this was only one clock, and also was not the newest generation of clock which is the principal component (PC) type horvath pan tissue clock (these newer versions are more accurate at n=1 levels). The intervention included a specific plant based diet, 30 mins exercise per day, lactobacillus probiotics, unstated ‘sleep optimisation’ interventions, and breathing relaxation exercises.
We hope that in sharing my data, others who are actively measuring and trying to reverse epigenetic age will share theirs as well. Hopefully too, their multi-epi-clock average per unit time age reversal will best mine and they’ll share how they’re achieving those results to accelerate progress in the field of rejuvenation.
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“Epigenetics studies how our behaviors and environment can cause changes that affect the way our genes work. Epigenetic changes are vital to normal biological functioning and can affect natural cycles of cellular death, renewal, and senescence. Different lifestyle and behavioral factors such as diet, sleep, exercise, smoking, and drinking alcohol can also affect the composition and location of the chemical groups that bind to our DNA. Environmental factors such as stress and trauma or even neighborhoods or zip codes may also have an impact.”
Epigenetic age can be measured independently of chronological age via different methods. Given that no single epigenetic clock has been clinically validated, and that there are accuracy issues with methodologies of some clocks, some trials have used the average reduction in multiple epigenetic age clocks. For example, the TRIIM trial (aimed at thymic rejuvenation) showed an average reduction in epigenetic age by 0.9 years at 9 months (0.1 years per month) across 4 epigenetic clocks (Horvath, Peno, Hannum, Grim).
Below we report results of 4.5 years epigenetic age reduction in 7 months across 6 epigenetic clocks, and 5.1 years if one includes the 7 months time passed (0.73 years per month). Notably these clocks include new next generation Principal Component Analysis (PC) clocks which yield 20x higher power than previous clocks (although PCGrimAge has an issue that it requires chrono age in the calculation, unlike the original GrimAge). Note that GrimAge has not yet been calculated from this dataset and will be soon.
Note that I, the subject, was already on 5 mg weekly low dose rapamycin for 1 year+ before the April measures, and the November read was before switching to 10 mg rapa biweekly in December. We will continue to publish updates on these 6 clock results, as well as GrimAge, approximately quarterly.
This was achieved without the use of growth hormone as used in the TRIIM trial.
PCHorvath 1 = Principal component Horvath pan-tissue clock
PCHorvath 2 = Principal component Horvath skin and blood clock
PCHannum = Principal component Hannum clock
PCPhenoage = Principal component PhenoAge clock
Age Horvath IEAA = Horvath Intrinsic Epigenetic Aging acceleration clock independent of blood counts converted to biological age
Age Hannum IEAA = Hannum Intrinsic Epigenetic Aging acceleration clock independent of blood counts converted to biological age
We also found telomeres epigenetic clock (high accuracy via principal component analysis) increased too during this time (units kilobase pairs, but this does not represent median telomere length directly, rather it is more akin to an assay unique scale of total telomere length per number of cells and hence more accurately represents the telomere histogram).
This represents a 10 year reduction in telomere length from age 50 to age 40. However, it is unclear what the within day, within month and within year intra assay coefficient of variation are on this assay, and exactly how it compares to standard 20th centile or median telomere length measures, which may suffer from +/- 33% random change within any individual across the space of 12 months.
