8 uses of MOPS buffer you didn’t know

forward from: http://www.hopaxfc.com

MOPS is a zwitterionic biological buffer often used in biological and biochemical research. After reviewing several academic papers in which MOPS buffer was studied, we would like to share with you some recommendations and concerns you must have when using this buffer. We hope you find these tips useful!

MOPS basic information:

• CAS Number: 1132–61–2
• Molecular Weight: 209.3
• Formula: C7H15NO4S
• Useful ph range: 6.5–7.9
• pKa (25°C): 7.0–7.4
• Price / Specifications: Click here

What is MOPS recommended for?

1. For denaturing gel electrophoresis of RNA(1)
2. For protein purification in chromatography
3. To measure absorption during UV/VIS spectrophotometry and to study redox properties using cyclic voltammetry(2)
4. In nitrogenase electron transfer mechanism(3)
5. In the separation of nucleic acids and proteins by electrophoresis(4)
6. In the control of culture media pH(5), including cell culture media for yeast, bacteria and mammalian cells.
7. It has been used as a buffer component of charcoal yeast extract medium(6)
8. It interacts with peptide backbone of bovine serum albumin leading to a net stabilization of the protein(7)

Which concerns should you have before choosing MOPS for your research?

• MOPS concentration should be not higher than 20mM when used for mammalian cell culture
• Although most studies found little complexation between Mops and metals, some studies found that interferences may occur due to metal complex formation(8)
• It can modify lipid interactions(9)
• MOPS can influence the thickness and barrier properties of rat endothelial surface layers(10)
• It interacts and forms complexes with DNA(11)
• It can slightly affect mRNA expression of in vitro produced bovine embryos(12)
• Mops interferes with Lowry protein determination
• It can be oxidized by H2O2, but since buffer oxidation is slow, no significant impact on biological systems is expected to happen(13)
• MOPS is partly degraded on autoclaving in the presence of glucose

Useful tips about MOPS:

• It does not interfere with Bradford or bicinchoninic acid assays(14)

Hopax MOPS Buffer

Hopax Fine Chemicals is among the largest producers of MOPS in the world. Our products are shipped daily to top research centers and biotech companies in Europe, America and Asia.

What we offer:

• Product straight from our manufacturing sites
• Worldwide shipping to your door
• Assistance with shipping
• Small and bulk packages (from grams to tons)
• International quality standards
• After-sales service with English speaking staff

References:

1 Russell, D. W. and Sambrook, J. (2001) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, 3rd edn. Available at https://www.cshlpress.com/pdf/sample/2013/MC4/MC4FM.pdf

2 Collins, C. A., Fry, F. H., Holme, A. L., Yiakouvaki, A., Al-Qenaei, A., Pourzand, C., & Jacob, C. (2005) Towards multifunctional antioxidants: synthesis, electrochemistry, in vitro and cell culture evaluation of compounds with ligand/catalytic properties. Organic & Biomolecular Chemistry, 3(8), 1541–1546. Available at https://pubs.rsc.org/en/content/articlelanding/2005/ob/b503282m#!divAbstract

3 Mayweather, D., Danyal, K., Dean, D. R., Seefeldt, L. C. and Hoffman, B. M. (2012) Biochemistry, 51, 8391–8398. Available at https://www.ncbi.nlm.nih.gov/pubmed/23050654

4 Garfin, D. E. (1990) in Methods in Enzymology, Guide to protein purification, ed. P. M. Deutsche, Academic Press, San Diego, pp. 425–441. Available at https://www.worldcat.org/title/guide-to-protein-purification/oclc/463300660

5 de Klerk, G. J., Hanecakova J. and Jasik, J. (2008) Plant Cell, Tissue Organ Cult., 95, 285–292. Available at https://www.researchgate.net/publication/226844998_Effect_of_medium-pH_and_MES_on_adventitious_root_formation_from_stem_disks_of_apple

6 Edelstein, P. H. and Edelstein, M. A. C. (1993) J. Clin. Microbiol., 31, 3329–3330. Available at https://jcm.asm.org/content/jcm/31/12/3329.full.pdf

7 Taha, M., Gupta, B. S., Khoiroh, I., Lee, M.J. (2011) Interactions of Biological Buffers: The Ubiquitous “Smart” Polymer PNIPAM and the Biological Buffers, MES, MOPS and MOPSO. Macromolecules, 44, 8575–8589. Available at https://pubs.acs.org/doi/abs/10.1021/ma201790c

8 Taha, M., Gupta, B. S. and Lee, M. J. (2011) Chem. Eng. Data, 56, 3541–3551. Available https://pubs.acs.org/doi/abs/10.1021/je200345a

9 Koerner, M. M., Palacio, L. A., Wright, J. W., Schweitzer, K. S., Ray, B. D. and Petrache, H. I. (2011) Biophys. J.,101, 362–369. Available https://www.ncbi.nlm.nih.gov/pubmed/21767488

10 van Haaren, P. M. A., VanBavel, E., Vink, H. and Spaan, J. A. E. (2005) J. Physiol.: Heart Circ. Physiol., 289, H2503–H2507. Available at https://pdfs.semanticscholar.org/6bb2/3e5704e2e54e798ceda40630c797089909ad.pdf

11 Stellwagen, N. C., Bossi, A., Gelfi, C. and Righetti, P. G. (2000) Anal. Biochem., 287, 167–175. Available at https://www.ncbi.nlm.nih.gov/pubmed/11078596

12 Palasz, A. T., Breña, P. B., De la Fuente, J. and Gutiérrez- Adán, A. (2008) Theriogenology, 70, 1461–1470. Available http://europepmc.org/abstract/MED/18675448

13 Zhao, G. and Chasteen, N. D. (2006) Anal. Biochem., 349, 262–267. Available at https://www.ncbi.nlm.nih.gov/pubmed/16289439

14 Grady, J. K., Chasteen, N. D., & Harris, D. C. (1988) Radicals from “Good’s” buffers. Analytical Biochemistry, 173(1), 111–115. Available at https://www.ncbi.nlm.nih.gov/pubmed/2847586