PCR Technique — Polymerase Chain Reaction
DNA Amplification
DNA ( Deoxyribose Nucleic Acid) is the genetic material. It helps to transfer genes from one generation to another generation. Many experiments are based on DNA. So its amplification is necessary for research for future use of DNA sample for testing.
PCR i.e. Polymerase Chain Reaction is a technique to amplify the DNA. It is an automated machine. This technique helps to generate large quantities of specified DNA. It is an amplification technique that can generate a billion copies of specified DNA.
Karry Mullis in 1984 developed a PCR technique. It is a basic technique in the molecular biology field. The principle of PCR technique based on three steps -
1. Denaturation
2. Renaturation
3. Synthesis or polymerases
The technical explanation of PCR step by step Requirements
1.Target DNA 100–35,000 bp in length
2. Two primers 17–30 nucleotide in length
3. Four relevant deoxynucleotides
