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Micropropagation- In vitro propagation

The miniature version of conventional propagation


Plant tissue culture is used to maintain or grow plant cells by in vitro cultivation under aseptic and controlled environment conditions. In short plant tissue culture is a collection of techniques used for the production of virus-free plants, for the production of artificial seeds and trangenic pants, for the varietal improvements of plants, and many more.

In other terms, it is an experimental technique through which callus ( a mass of cells) is produced from explant cells. Tissue culture refers to the growth of organs, tissues, and cells in an artificial medium.

Gottlieb Haberlandt considered a father of plant tissue culture because he suggested that “ one could successfully cultivate artificial embryos from vegetative cells”.The idea of Totipotency was conceived by him. He was the first to culture isolated, fully differentiated cells in a nutrient medium.

Micropropagation is a modern plant tissue culture methods used to rapidly multiplying stock plant material to produce many progeny plants. It is an essential technique for the plant breeding program. It is the fastest method of asexual reproduction in comparison to propagation through seeds.

One of the most important applications of plant tissue culture is the micropropagation of plant species and it has attained the status of a large plant-based industry.

Micropropagation such a promising technique to obtain genetically pure elite populations under in vitro conditions. This technique is a miniature version of conventional propagation. Micropropagation of plants through tissue culture technique contributed significantly to the enhanced production of high-quality planting material.

It has now come to stay as an important technique for the rapid production of many commercially important plants like Gladiolus, Eucalyptus, Gerbera, Dalbergia, Freesia, and several important fruits trees and medicinal plants.

Major Steps In micropropagation

  1. Selection of healthy disease-free mother plant for culture initiation.
  2. Establishment of aseptic culture.
  3. Shoot multiplication by using a defined culture medium.
  4. Rooting of regenerated shoots in vitro and germination of somatic embryos.
  5. Shoots are separated manually from clusters and transferred in a rooting medium.
  6. Acclimatization or hardening by transfer of plantlets to the natural environment.

Advantages of Micropropagation

  1. Allows rapid and large-scale production of desired horticulture varieties.
  2. Helps to produce genetically uniform plants i.e. clones and development of genetically transformed plants I,e, transgenics are be produced with the help of this technique.
  3. Allow rapid multiplication.
  4. Provides a reliable and economical method for maintaining pathogen-free plants.
  5. Plant multiplication can continue throughout the year irrespective of seasons.
  6. Multiplication of plants can occur in a small space in a tissue culture lab because each flask can accommodate hundreds of plantlets.
  7. Micropropagation facilitates the international exchange of germplasm without the inherent risk of spreading diseases and pathogens.
  8. Stocks of germplasm can be maintained for many years.
  9. Plants with very small seeds like orchids are propagated from seed in sterile culture.
  10. Useful to produce sterile plants that do not produce viable seeds.

Micropropagation is true to the type of propagation of selected genotypes using small pieces of tissues which can be used to raise hundreds and thousands of plants in a continuous process.

References :

Bhojwani, S.S. and Razdan, M.K. 1982.Plant Tissue Culture: Theory and Practice. Elsevier Sci.Publ. Amsterdam .p1–520

NE Borloung, Plant Physiology 124(2),487–490,2000

Originally published at on September 25, 2020.



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