Helping sperm to reach the egg
Researchers have identified two new components in a channel protein that allows sperm to move more efficiently.
Male mammals ejaculate millions of sperm cells each time they mate with a female. Only a few of these cells manage to travel up the female’s reproductive tract to reach the egg, and usually only one sperm fertilizes it. Freshly ejaculated sperm are incapable of fertilizing eggs and have to undergo several changes within the female to become able to do so. One crucial change occurs in the sperm tail, which starts to beat vigorously in a whip-like motion. This type of movement — known as hyperactivated motility — enables the sperm to swim towards the egg, push through a sticky coating that surrounds it, and then burrow into it.
Hyperactivated motility is triggered when calcium ions enter the sperm cell via a specific channel protein known as CatSper, which is found in the membrane that surrounds the cell. CatSper channels form groups (known as complexes) with several other proteins that are arranged in a unique pattern of four straight ‘stripes’ running down the tail of the sperm. This arrangement is necessary for hyperactivated motility and mutations in the genes that encode these proteins can lead to infertility in males. The CatSper channel complex is known to contain seven proteins: four that form a pore through which calcium ions can enter, and three accessory proteins whose roles in hyperactivated motility are less clear.
Jean-Ju Chung and colleagues identified two genes in mice that encode new accessory proteins in the CatSper channel complex named CatSper epsilon and CatSper zeta. Further experiments analyzed the role of CatSper zeta in more detail. Mutant males that lack CatSper zeta have fragmented patterns of CatSper stripes in the tails of their sperm. Moreover, fewer calcium ions were able to pass through the channels to enter the cell. Together, this made the sperm tail more rigid, which prevented it from moving efficiently within the female, resulting in reduced fertility. Chung and colleagues also found that the mutant sperm were less able to penetrate the egg than normal sperm.
During evolution, the gene that encodes CatSper zeta appeared first in mammals and may represent an adaptation that improved the chances of a sperm fertilizing the egg inside the reproductive tract of female mammals. Future challenges will be to explore how the CatSper channel assembles on the membrane of sperm and find out exactly how calcium ions trigger hyperactivated motility.
To find out more
Read the eLife research paper on which this eLife digest is based: “CatSperζ regulates the structural continuity of sperm Ca2+ signaling domains and is required for normal fertility” (February, 23 2017).
