Which view is objective?

When I started this project I tried out a lot of different ways of optical staining, al these ways of optical staining have there pro’s and con’s. But which of these used techniques is objective and is showing the real truth? Are there more truths?

This question is still going trough my mind when working on this project cause when people ask me wow is this real? What is the right answer? What answer are they looking for? Does it matter which one is real?

Pick me!

When it comes to “crating a curated reality” under the microscope there are a a lot of options, all resulting in a different reality.

The images below show the results with the information about the technique used in the top right corner.

To get good results showing the differences in techniques I used the following compound in my sample.

2,3-dihydroxybutanedioic acid
Bright field illumination, sample contrast comes from absorbance of light in the sample.
Oblique illumination, the images has the appearance of a tree-dimensional object under very oblique illumination, causing strong light and dark shadows on the corresponding faces.
Dark field illumination, sample contrast comes from light scattered by the sample.
Cross-polarized illumination, sample contrasts comes from the rotation of polarized light trough the sample.
Cross-polarized + Berek filter illumination, sample contrast comes from the rotation of the polarised light trough the sample “and optically coloured with a half wave length filter”.

If it comes to science, there would probably be “one” right answer according to the regulations. But in this case I guess its all about form and taste.

Talking about form and taste what would be your 1st pick?

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