Finding Differentiating Isoforms in Bone Marrow Subpopulations: an interview with Anne

Liz Tseng
Liz Tseng
Apr 15 · 7 min read
Overview of normal human hematopoiesis. Drawing by A. Rad. Source from wikipedia.
Figure 1 from Mays et al. showing the experimental setup. Healthy bone marrow discarded from the hospital was segregated into lineage positive and negative populations using antibodies. Sequencing was done on both PacBio Iso-Seq (full-length cDNA) and Illumina (fragmented cDNA). Predicted protein sequences based on the Iso-Seq data were validated by mass spectrometry later.

A log of Anne retrieving the left-over material from bone marrow collections at the MedStar Georgetown University Hospital Cell Processing Unit. She recovered the samples from the waste and isolated the bone marrow cell populations herself!
Figure 5 from Mays et al. showing short read assemblies (gray triangles) fail to reveal the increasing splicing complexity as the number of exons increase as full-length Iso-Seq (black circles) does.
Figure 5 from Mays et al. confirming the expression of the HLA genes through mass spectrometry.

PacBio

Short reads on outstanding genetics projects made possible by long-read sequencing

Liz Tseng

Written by

Liz Tseng

All things RNA. Food lover of Taiwanese origin. Trains in circus arts (rope and straps). Opinions are my own.

PacBio

PacBio

Short reads on outstanding genetics projects made possible by long-read sequencing