101 Tips

Welcome to 101 Tips, where we’ll share tips, tricks and historical info related to raising Zebrafish in the lab. Why 101? Seems like a lofty and reasonable goal, we’ll slowly post these and continue to share the most pertinent information available.

Tip 1:

Did you know that rotifers were once considered a pest in traditional Japanese aquaculture do to their rapid generation time in eel rearing ponds? Now these small critters (80–300 microns) are key in zebrafish labs and marine aquaculture hatcheries.

Reference: Guillaume, J., Kaushik, S., Bergot, P. and Metailler, R. (1999). Nutrition and Feeding of Fish and Crustaceans. Springer-Praxis. Pp. 215.

Tip 2:

What are the dissolved oxygen requirements for laboratory zebrafish and why is it important?

Currently the optimal dissolved oxygen level of laboratory zebrafish has not yet been determined. It is recommended that DO2 levels be maintained at or just under saturation (~7.8 mg/L at 28.0°C) based on their high metabolic rates as a small-bodied warm water species.


Boyd, C.E., 1979. Water Quality in Warmwater Fish Ponds. Alabama Agricultural Experiment Station, Auburn University, Alabama. 259. Pp.

Helfman, G., Collette, B., Facey, D. 1997. The Diversity of Fishes. Blackwell Science, Malden, MA. 528 pp.

Lawrence, C. The husbandry of zebrafish (Danio rerio): A review. Aquaculture 269, 6–7.

Timmons, M.B., Ebeling, J.M., Wheaton, J.M., Summerelt, S.T., Vinci, B.J. (Eds.), 2002. Recirculating Aquaculture Systems, 2nd ed. Cayuga Aqua Ventures, Ithaca, N.Y. 757 pp.

danio lab·
1 min
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