Microscopy

Welcome to my two-part tutorial on image segmentation and analysis for microscopy (and beyond). Using OpenCV, Scipy and Scikit Image in Python scripting, you will be able to quickly segment thousands of cells and extract intensity data for statistical analysis. In this tutorial, you will learn how to segment images…

If you’re a biologist who wants to study cells, you may sometimes have to count a bunch of “blobs” that are found randomly in the cell in the 3D space. We would call those bodies foci. There are sometimes plugins like in FIJI or specialized software like Zen 2 from…

Welcome to the last of the 3 tutorials on foci detection in OpenCV for life scientists. Previously, we learned how to extract pixel coordinates and intensity values, and generated artificial 3D images to work with for this tutorial on using machine learning to detect foci numbers, location and size in…

Hello, and welcome to the second tool in the pipeline to count foci — the foci generator. This tool can be used for generating test data for other pipelines in biology, like generating random foci (organelles, membranelles bodies, vacuoles, etc.) occurring in cells. In the previous tutorial, we extracted pixel…

Welcome to the cutting edge of microscopy! In this blog, we will explore a groundbreaking discovery made by researchers at Caltech, who have harnessed the power of quantum entanglement to double the resolution of light microscopes. This breakthrough, known as Quantum Microscopy by Coincidence (QMC), has the potential to…

We are thrilled to announce the publication of exciting new research using Ramona’s Multi-Camera Array Microscope (MCAM) technology in Nature Photonics. Parallelized computational 3D video microscopy of freely moving organisms at multiple gigapixels… Wide-field-of-view microscopy that can resolve three-dimensional (3D) information at high speed and spatial resolution…www.nature.com This paper discusses the existing challenges of imaging freely-moving small model organisms, such as zebrafish and fruit flies, across multiple spatial scales, and explores how Ramona’s MCAM technology can be used to overcome current limitations by capturing high-speed 3D topographic videos over a large area.

Abstract Gastric schwannomas are unusual and account for only 0.2% of all gastric tumors. Given their rarity and the absence of randomized trials, the diagnostic and therapeutic burden of these tumors remains difficult. Like the latter, and despite multimodal treatment, their prognosis remains unfavorable. …

Microscopy has been a passion of mine for as long as I can remember. From the first time I looked through a microscope, I was captivated by the beauty and complexity of the microscopic world. It’s a fascinating world, full of intricate details and endless mysteries waiting to be discovered. …

The visualization, sharing and annotation capabilities of WEBKNOSSOS have grown a lot over the last year. In this article, we will be revisiting some of our favorite features. Have look and make sure you have not missed anything! The interpolation feature Annotate a cell, skip a few slices and annotate it again. Use…